Catalog #CP050
RecombiMAb anti-mouse CD106 (VCAM-1)
Clone
M/K-2.7-CP050
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
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Product Description
The M/K-2.7-CP050 monoclonal antibody is a chimeric version of the original M/K-2.7 antibody. The variable domain sequences are identical to the original M/K-2.7 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The M/K-2.7-CP050 monoclonal antibody reacts with mouse CD106 also known as VCAM-1 and INCAM-110. CD106 is a 110 kDa single chain type I glycoprotein that is expressed primarily on activated vascular endothelial cells but has also been reported on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. CD106 expression is induced by inflammatory stimuli and cytokines. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 which contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Stromal cells from mouse bone marrow |
| Reported Applications |
in vivo VCAM-1 neutralization* Immunofluorescence* *Reported for the original rat IgG1 M/K-2.7 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |