Catalog #CP101
RecombiMAb anti-mouse CCR5 (CD195) (LALA-PG)
Clone
C5Mab-2-CP101
Reactivities
Mouse
Isotype
Mouse IgG2a (LALA-PG), κ
(switched from Rat Ig2b, κ)
(switched from Rat Ig2b, κ)
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Product Description
The C5Mab-2-CP101 monoclonal antibody is a recombinant, chimeric version of the original C5Mab-2 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, κ to mouse IgG2a, κ for use in murine models. Additionally, C5Mab-2-CP101 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). The LALA-PG variant has demonstrated significantly reduced effector function, C1q binding and C3 fixation compared to other common silencing mutations such as the LALA and DANG variants while retaining favorable biophysical and manufacturing properties. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The C5Mab-2 monoclonal antibody reacts with mouse CC chemokine receptor type-5 (CCR5), also known as MCP-1 receptor, CKR5, and CD192. CCR5 is a seven-pass transmembrane protein from the GPCR family, and is expressed on dendritic cells (DCs), T cells (including Tregs), macrophages, eosinophils, NK cells, myeloid-derived suppressor cells (MDSCs), and some non-hematopoietic cells such as epithelial cells, endothelial cells, fibroblasts (including vascular smooth muscle), and microglia. The natural agonistic, or activating, ligands of CCR5 include CCL3/MIP-1 alpha, CCL3L1, CCL4 (MIP-1 beta), CCL5/RANTES, CCL8, CCL11, CCL13, and CCL16. Notably, CCL7/MCP-3 binds to CCR5 as a natural antagonist/deactivating ligand, thereby blocking receptor signaling and influencing inflammation and immune responses in a complex and context-dependent manner. CCR5 receptor activation regulates the migration and activation of lymphocytes, immune surveillance, tumorigenesis, and inflammation, including the pathogenesis of inflammatory diseases. To facilitate the recruitment of the immune cells to inflammation sites, CCR5 directs the process of chemotaxis (immune cell migration) along the chemokine gradient. In neurobiology, CCR5 acts as a suppressor of learning, memory, neuronal plasticity, and synaptic connections in the brain. CCR5 is well documented to act as a common coreceptor for HIV-1, and CCR5 antagonists are reported to provide protection against HIV-1. In vivo studies with mouse models of traumatic brain injury (TBI) have linked CCR5 knockdown to reduced learning deficit and improvement of cognitive function. In view of the pleiotropic effects of CCR5, its antagonists, including monoclonal antibodies, have emerged as promising tools for experimental therapeutics of tumors and inflammatory diseases.
The C5Mab-2 monoclonal antibody reacts with mouse CC chemokine receptor type-5 (CCR5), also known as MCP-1 receptor, CKR5, and CD192. CCR5 is a seven-pass transmembrane protein from the GPCR family, and is expressed on dendritic cells (DCs), T cells (including Tregs), macrophages, eosinophils, NK cells, myeloid-derived suppressor cells (MDSCs), and some non-hematopoietic cells such as epithelial cells, endothelial cells, fibroblasts (including vascular smooth muscle), and microglia. The natural agonistic, or activating, ligands of CCR5 include CCL3/MIP-1 alpha, CCL3L1, CCL4 (MIP-1 beta), CCL5/RANTES, CCL8, CCL11, CCL13, and CCL16. Notably, CCL7/MCP-3 binds to CCR5 as a natural antagonist/deactivating ligand, thereby blocking receptor signaling and influencing inflammation and immune responses in a complex and context-dependent manner. CCR5 receptor activation regulates the migration and activation of lymphocytes, immune surveillance, tumorigenesis, and inflammation, including the pathogenesis of inflammatory diseases. To facilitate the recruitment of the immune cells to inflammation sites, CCR5 directs the process of chemotaxis (immune cell migration) along the chemokine gradient. In neurobiology, CCR5 acts as a suppressor of learning, memory, neuronal plasticity, and synaptic connections in the brain. CCR5 is well documented to act as a common coreceptor for HIV-1, and CCR5 antagonists are reported to provide protection against HIV-1. In vivo studies with mouse models of traumatic brain injury (TBI) have linked CCR5 knockdown to reduced learning deficit and improvement of cognitive function. In view of the pleiotropic effects of CCR5, its antagonists, including monoclonal antibodies, have emerged as promising tools for experimental therapeutics of tumors and inflammatory diseases.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 8.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | Mouse CCR5 expressing CHO-K1 cells |
| Reported Applications |
Flow cytometry For details on in vivo applications please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 8.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation* |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |
* Additional quality control measures for our InVivoPlus™ products include advanced binding validation, murine pathogen screening, protein aggregation screening, and ultra-low endotoxin levels. The superior quality of our InVivoPlus™ products will meet and exceed the strict demands and rigorous standards required for in vivo research. Learn more about the InVivoPlus™ difference here.