Catalog #CP102
RecombiMAb anti-mouse CCR4
Clone
C4Mab-1-CP102
Reactivities
Mouse
Isotype
Mouse IgG2a, κ (switched from Rat IgG1, κ)
Product Description
The C4Mab-1-CP102 monoclonal antibody is a chimeric version of the original C4Mab-1 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG1, κ to Mouse IgG2a, κ. The C4Mab-1-CP102 antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
C4Mab-1-CP102 binds to mouse CCR4 (C-C Chemokine Receptor 4), a G protein-coupled receptor encoded by the Ccr4 gene primarily expressed on Th2-polarized CD4+ T cells, regulatory T cells (Tregs), invariant NKT cells, skin-homing T cells (Trm), and some innate immune cells like macrophages and NK cells. CCR4 is the main receptor for CCL17 (TARC) and CCL22 (MDC), which drive chemotaxis to inflamed tissues, lymphoid organs, and skin. Ligand binding activates Gαi-mediated pathways, inhibiting cAMP, mobilizing Ca²⁺, and triggering migration via PI3K, MAPK (p38/JNK), and cytoskeletal changes. Unlike human CCR4, mouse CCR4 also binds CKLF1. CCR4 plays an important role in several disease areas including allergic and atopic diseases, autoimmune and neuroinflammatory diseases, as well as cancer and hematologic malignancies. Epitope mapping has shown that mouse CCR4 residues Thr7, Asp8, and Gln11 are essential for C4Mab-1 antibody binding and that Val6, Thr9, and Thr10 are involved in C4Mab-1 binding. These results indicate that the critical binding epitope of C4Mab-1 includes Thr7, Asp8, and Gln11 of mouse CCR4 [1].
1: Asano, Teizo et al. “Identification of the Binding Epitope of an Anti-mouse CCR4 Monoclonal Antibody, C4Mab-1.” Monoclonal antibodies in immunodiagnosis and immunotherapy vol. 41,4 (2022): 214-220. doi:10.1089/mab.2022.0015
C4Mab-1-CP102 binds to mouse CCR4 (C-C Chemokine Receptor 4), a G protein-coupled receptor encoded by the Ccr4 gene primarily expressed on Th2-polarized CD4+ T cells, regulatory T cells (Tregs), invariant NKT cells, skin-homing T cells (Trm), and some innate immune cells like macrophages and NK cells. CCR4 is the main receptor for CCL17 (TARC) and CCL22 (MDC), which drive chemotaxis to inflamed tissues, lymphoid organs, and skin. Ligand binding activates Gαi-mediated pathways, inhibiting cAMP, mobilizing Ca²⁺, and triggering migration via PI3K, MAPK (p38/JNK), and cytoskeletal changes. Unlike human CCR4, mouse CCR4 also binds CKLF1. CCR4 plays an important role in several disease areas including allergic and atopic diseases, autoimmune and neuroinflammatory diseases, as well as cancer and hematologic malignancies. Epitope mapping has shown that mouse CCR4 residues Thr7, Asp8, and Gln11 are essential for C4Mab-1 antibody binding and that Val6, Thr9, and Thr10 are involved in C4Mab-1 binding. These results indicate that the critical binding epitope of C4Mab-1 includes Thr7, Asp8, and Gln11 of mouse CCR4 [1].
1: Asano, Teizo et al. “Identification of the Binding Epitope of an Anti-mouse CCR4 Monoclonal Antibody, C4Mab-1.” Monoclonal antibodies in immunodiagnosis and immunotherapy vol. 41,4 (2022): 214-220. doi:10.1089/mab.2022.0015
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | mCCR4 N-terminal peptide immunization |
| Reported Applications |
Flow Cytometry* *Reported for the original C4Mab-1 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation* |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |
* Additional quality control measures for our InVivoPlus™ products include advanced binding validation, murine pathogen screening, protein aggregation screening, and ultra-low endotoxin levels. The superior quality of our InVivoPlus™ products will meet and exceed the strict demands and rigorous standards required for in vivo research. Learn more about the InVivoPlus™ difference here.