RecombiMAb anti-mouse 4-1BBL (CD137L) (D265A)

Catalog #CP039
Clone:
TKS-1-CP039
Reactivities:
Mouse
Isotype:
Mouse IgG1
(switched from rat IgG2a)

$500.00

Choose an Option...
  • 1 mg - $500.00
  • Custom Amount (Quotes Only)
In stock
Only %1 left

Product Details

The TKS-1-CP039 monoclonal antibody is a chimeric version of the original TKS-1 antibody. The variable domain sequences are identical to the original TKS-1 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The TKS-1-CP039 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The TKS-1-CP039 monoclonal antibody reacts with mouse 4-1BB ligand (4-1BBL), a type II transmembrane glycoprotein also known as CD137L. 4-1BBL is a 97 kDa member of the TNF superfamily and is expressed by dendritic cells, macrophages, and activated B and T lymphocytes. Interaction of 4-1BBL with 4-1BB (CD137) provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF-κB, c-Jun and p38 downstream pathways. The TKS-1 antibody has been shown to inhibit the binding of soluble 4-1BB to 4-1BBL in vitro.

Specifications

Isotype Mouse IgG1, κ
Recommended Isotype Control(s) RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Mutations D265A
Immunogen Mouse 4-1BBL transfected NRK cells
Reported Applications in vivo 4-1BBL blockade*
ELISA*
*Reported for the original rat IgG2a TKS-1 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

See the references for the original rat IgG2a TKS-1 antibody (https://bioxcell.com/catalogsearch/result/?q=BE0110).