Name: RecombiMAb anti-mouse 4-1BB (CD137) (LALA-PG)
Brand: CP038
SKU: CP038
Availability: InStock

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Technical Data Sheet Safety Data Sheet CoA

Product Details

The LOB12.3-CP038 monoclonal antibody is a chimeric version of the original LOB12.3 antibody. The variable domain sequences are identical to the original LOB12.3 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The LOB12.3-CP038 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The LOB12.3-CP038 antibody reacts with mouse 4-1BB, a TNF receptor superfamily member also known as CD137. 4-1BB is a 39 kDa transmembrane protein expressed by T lymphocytes, NK cells, dendritic cells, granulocytes, and mast cells. Upon binding its ligand 4-1BBL, 4-1BB provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF- κB, c-Jun and p38 downstream pathways. The importance of the 4-1BB pathway has been underscored in a number of diseases, including cancer. Agonistic anti-4-1BB antibodies have been reported to induce T cell mediated antitumor immunity. The LOB12.3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling and delay tumor growth in vivo when administered in combination with immune checkpoint inhibitors.

Specifications

Isotype	Mouse IgG2a, κ
Recommended Isotype Control(s)	RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Mutations	LALA-PG
Immunogen	Mouse CD137 human Fc fusion protein
Reported Applications	in vivo activation of 4-1BB* *Reported for the original rat IgG1 LOB12.3 antibody
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from HEK293 cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Murine Pathogen Tests	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.