Catalog #SIM0040
InVivoSIM anti-human mesothelin (Amatuximab Biosimilar)
Clone
Amatuximab
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Amatuximab, making it ideal for research use. Amatuximab is a high-affinity chimeric monoclonal antibody that reacts with mesothelin (MSLN). Human MSLN is synthesized as a 71-kDa precursor that translocates to the cell surface, where it is cleaved into 31-kDa megakaryocyte potentiating factor (MPF) and 40-kDa mature MSLN protein. MSLN is a glycosylphosphatidyl inositol (GPI)-anchored membrane glycoprotein whose expression in adult humans is restricted to mesothelium. Notably, MSLN is found over-expressed in many epithelial cancers, such as epithelial mesotheliomas, ovarian cancers, lung adenocarcinomas, and pancreatic ductal adenocarcinomas. MSLN interacts with cancer antigen 125 (CA125), a specific epitope expressed on MUC16, and it is suggested to play a role in tumorigenesis and metastasis. The tumor-specific expression of MSLN makes it an attractive target antigen for cancer immunotherapy. Amatuximab has been shown to elicit antibody-dependent cellular cytotoxicity (ADCC) against MSLN+ cancer cell lines in vitro, and a greater efficacy in mouse tumor xenografts when combined with chemotherapy.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human mesothelin |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |