InVivoSIM anti-human mesothelin (Amatuximab Biosimilar)
Catalog #SIM0040
Clone:
Amatuximab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Amatuximab, making it ideal for research use. Amatuximab is a high-affinity chimeric monoclonal antibody that reacts with mesothelin (MSLN). Human MSLN is synthesized as a 71-kDa precursor that translocates to the cell surface, where it is cleaved into 31-kDa megakaryocyte potentiating factor (MPF) and 40-kDa mature MSLN protein. MSLN is a glycosylphosphatidyl inositol (GPI)-anchored membrane glycoprotein whose expression in adult humans is restricted to mesothelium. Notably, MSLN is found over-expressed in many epithelial cancers, such as epithelial mesotheliomas, ovarian cancers, lung adenocarcinomas, and pancreatic ductal adenocarcinomas. MSLN interacts with cancer antigen 125 (CA125), a specific epitope expressed on MUC16, and it is suggested to play a role in tumorigenesis and metastasis. The tumor-specific expression of MSLN makes it an attractive target antigen for cancer immunotherapy. Amatuximab has been shown to elicit antibody-dependent cellular cytotoxicity (ADCC) against MSLN+ cancer cell lines in vitro, and a greater efficacy in mouse tumor xenografts when combined with chemotherapy.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human mesothelin |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
