Catalog #SIM0040
InVivoSIM anti-human mesothelin (Amatuximab Biosimilar)
Clone
Amatuximab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
Western blot
Flow cytometry
in vitro functional assays
ELISA
Western blot
Flow cytometry
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Amatuximab, making it ideal for research use. Amatuximab is a high-affinity chimeric monoclonal antibody that reacts with mesothelin (MSLN). Human MSLN is synthesized as a 71-kDa precursor that translocates to the cell surface, where it is cleaved into 31-kDa megakaryocyte potentiating factor (MPF) and 40-kDa mature MSLN protein. MSLN is a glycosylphosphatidyl inositol (GPI)-anchored membrane glycoprotein whose expression in adult humans is restricted to mesothelium. Notably, MSLN is found over-expressed in many epithelial cancers, such as epithelial mesotheliomas, ovarian cancers, lung adenocarcinomas, and pancreatic ductal adenocarcinomas. MSLN interacts with cancer antigen 125 (CA125), a specific epitope expressed on MUC16, and it is suggested to play a role in tumorigenesis and metastasis. The tumor-specific expression of MSLN makes it an attractive target antigen for cancer immunotherapy. Amatuximab has been shown to elicit antibody-dependent cellular cytotoxicity (ADCC) against MSLN+ cancer cell lines in vitro, and a greater efficacy in mouse tumor xenografts when combined with chemotherapy.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (E356D/M358L) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human mesothelin |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| RRID | AB_3696379 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |