InVivoSIM anti-human IL-6R (Sarilumab Biosimilar)
Catalog #SIM0085
Clone:
Sarilumab
Reactivities:
Human, Monkey
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Sarilumab, making it ideal for research use. Sarilumab is a humanized IgG1 monoclonal antibody that reacts with the interleukin 6 receptor (IL-6R), also known as CD126, IL6RA, gp80, IL6Q, and HIES5. IL-6R is an 80 kDa potent pleiotropic type I cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL-6R is expressed by activated T and B lymphocytes, monocytes, hepatocytes, plasma cells, and retinal endothelial cells. The IL-6R Ī± chain binds IL-6 but requires complexing with gp130 to initiate signaling pathways (e.g., the Jak/STAT and the MAPK pathways). The IL-6R complex is involved in antigen-specific immune responses, inflammatory responses, and neuronal development and is a major mediator of the acute phase reaction. Monoclonal antibodies, such as Sarilumab, are useful for blocking IL-6R activation in in vitro and in vivo research experiments by binding to and neutralizing the function of IL-6R. Sarilumab is reported to bind monomeric IL-6R and dimeric IL-6R Fc-fusion protein with high affinity (over 15x higher than tocilizumab). Sarilumab is reactive against human and cynomolgus monkey species, but it does not bind mouse IL-6R.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human IL-6R |
| Reported Applications |
In vivo functional assays in vitro functional assays Flow cytometry Western blot ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer