Catalog #SIM0049
InVivoSIM anti-human IL-1β (Gevokizumab Biosimilar)
Clone
Gevokizumab
Reactivities
Human, Monkey
Isotype
Human IgG2, κ
Product Description
This biosimilar antibody uses the same variable regions as the therapeutic antibody Gevokizumab, making it ideal for research use. Gevokizumab is a fully humanized monoclonal antibody that reacts with human IL-1β, a potent pro-inflammatory cytokine produced primarily by monocytes. Inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1 β, and after inflammasome assembly-mediated CASP1 activation, pro-IL1 is processed to generate active cytokine for secretion. IL-1β is an important mediator of the inflammatory response and is involved in a variety of cellular activities, including cell proliferation, differentiation, apoptosis, and T and B lymphocyte activation, neutrophil influx and activation, cytokine production, antibody production, fibroblast proliferation, and the synthesis of collagen as well as prostaglandins. IL-1β promotes Th17 differentiation of T-cells and also shows synergism with IL-12 to induce IFN-γ synthesis from Th1 cells. The Gevokizumab antibody binds a unique IL-1β epitope that is proximal to, but does not overlap with, the receptor/ligand interface, and this interaction reduces the binding of IL-1 to its receptor, i.e., IL-1 receptor type I (IL-1RI or CD121a). Gevokizumab neutralizes the human IL-1β by binding to it, and this antibody is used in preclinical research involving in vitro and in vivo experimental models of type 1 diabetes mellitus, inflammation, rheumatoid arthritis, cardiovascular biology, and cancer immunotherapy.
Specifications
| Isotype | Human IgG2, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human IL-1β |
| Reported Applications |
in vivo neutralization of human IL1β in vitro neutralization of human IL1β ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |