Catalog #SIM0049
InVivoSIM anti-human IL-1β (Gevokizumab Biosimilar)
Clone
Gevokizumab
Reactivities
Human, Monkey
Applications
in vivo neutralization of human IL1β
in vitro neutralization of human IL1β
ELISA
Western blot
Flow cytometry
in vitro neutralization of human IL1β
ELISA
Western blot
Flow cytometry
Isotype
Human IgG2, κ
Product Description
This biosimilar antibody uses the same variable regions as the therapeutic antibody Gevokizumab, making it ideal for research use. Gevokizumab is a fully humanized monoclonal antibody that reacts with human IL-1β, a potent pro-inflammatory cytokine produced primarily by monocytes. Inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1 β, and after inflammasome assembly-mediated CASP1 activation, pro-IL1 is processed to generate active cytokine for secretion. IL-1β is an important mediator of the inflammatory response and is involved in a variety of cellular activities, including cell proliferation, differentiation, apoptosis, and T and B lymphocyte activation, neutrophil influx and activation, cytokine production, antibody production, fibroblast proliferation, and the synthesis of collagen as well as prostaglandins. IL-1β promotes Th17 differentiation of T-cells and also shows synergism with IL-12 to induce IFN-γ synthesis from Th1 cells. The Gevokizumab antibody binds a unique IL-1β epitope that is proximal to, but does not overlap with, the receptor/ligand interface, and this interaction reduces the binding of IL-1 to its receptor, i.e., IL-1 receptor type I (IL-1RI or CD121a). Gevokizumab neutralizes the human IL-1β by binding to it, and this antibody is used in preclinical research involving in vitro and in vivo experimental models of type 1 diabetes mellitus, inflammation, rheumatoid arthritis, cardiovascular biology, and cancer immunotherapy.
Specifications
| Isotype | Human IgG2, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human IL-1β |
| Reported Applications |
in vivo neutralization of human IL1β in vitro neutralization of human IL1β ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |