Catalog #SIM0039
InVivoSIM anti-human CEACAM5 (Labetuzumab Biosimilar)
Clone
Labetuzumab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
Western blot
Flow cytometry
in vitro functional assays
ELISA
Western blot
Flow cytometry
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Labetuzumab, making it ideal for research use. This Labetuzumab biosimilar is a humanized anti-carcinoembryonic antigen antibody, and it reacts with carcinoembryonic antigen-related cell adhesion molecule 5 (i.e., CEACAM5), which is also called CEA or CD66e. During fetal or early embryonic stages, CEACAM5 is expressed in the digestive system, and it is found over-expressed in cancerous tissues. CEACAM5 acts as a cell adhesion molecule (CAM), and it inhibits anoikis, a type of apoptosis that is induced upon cell detachment from the ECM. Through this mechanism, CEACAM5 facilitates tumorigenesis and metastasis. CEACAM5 expression is often found upregulated in a variety of human epithelial malignancies, such as breast cancer, colon cancer, lung cancer, neuro-endocrine prostate cancer (NEPC), and pancreatic cancers. In cancer immunotherapy research, CEACAM5-targeted vaccines, bispecific T cell engagers (BiTEs), CAR T cell therapies, and antibody-drug conjugates are routinely tested in various experimental models. In vitro and in vivo studies involving the use of the humanized anti-carcinoembryonic antigen antibody Labetuzumab have shown the antibody to inhibit the cell proliferation and growth of CEACAM5+ tumors.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (K214R) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R |
| Immunogen | Human CEACAM5 |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| RRID | AB_3696378 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |