Catalog #SIM0039
InVivoSIM anti-human CEACAM5 (Labetuzumab Biosimilar)
Clone
Labetuzumab
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Labetuzumab, making it ideal for research use. This Labetuzumab biosimilar is a humanized anti-carcinoembryonic antigen antibody, and it reacts with carcinoembryonic antigen-related cell adhesion molecule 5 (i.e., CEACAM5), which is also called CEA or CD66e. During fetal or early embryonic stages, CEACAM5 is expressed in the digestive system, and it is found over-expressed in cancerous tissues. CEACAM5 acts as a cell adhesion molecule (CAM), and it inhibits anoikis, a type of apoptosis that is induced upon cell detachment from the ECM. Through this mechanism, CEACAM5 facilitates tumorigenesis and metastasis. CEACAM5 expression is often found upregulated in a variety of human epithelial malignancies, such as breast cancer, colon cancer, lung cancer, neuro-endocrine prostate cancer (NEPC), and pancreatic cancers. In cancer immunotherapy research, CEACAM5-targeted vaccines, bispecific T cell engagers (BiTEs), CAR T cell therapies, and antibody-drug conjugates are routinely tested in various experimental models. In vitro and in vivo studies involving the use of the humanized anti-carcinoembryonic antigen antibody Labetuzumab have shown the antibody to inhibit the cell proliferation and growth of CEACAM5+ tumors.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R |
| Immunogen | Human CEACAM5 |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |