InVivoSIM anti-human CEACAM5 (Labetuzumab Biosimilar)
Catalog #SIM0039
Clone:
Labetuzumab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Labetuzumab, making it ideal for research use. This Labetuzumab biosimilar is a humanized anti-carcinoembryonic antigen antibody, and it reacts with carcinoembryonic antigen-related cell adhesion molecule 5 (i.e., CEACAM5), which is also called CEA or CD66e. During fetal or early embryonic stages, CEACAM5 is expressed in the digestive system, and it is found over-expressed in cancerous tissues. CEACAM5 acts as a cell adhesion molecule (CAM), and it inhibits anoikis, a type of apoptosis that is induced upon cell detachment from the ECM. Through this mechanism, CEACAM5 facilitates tumorigenesis and metastasis. CEACAM5 expression is often found upregulated in a variety of human epithelial malignancies, such as breast cancer, colon cancer, lung cancer, neuro-endocrine prostate cancer (NEPC), and pancreatic cancers. In cancer immunotherapy research, CEACAM5-targeted vaccines, bispecific T cell engagers (BiTEs), CAR T cell therapies, and antibody-drug conjugates are routinely tested in various experimental models. In vitro and in vivo studies involving the use of the humanized anti-carcinoembryonic antigen antibody Labetuzumab have shown the antibody to inhibit the cell proliferation and growth of CEACAM5+ tumors.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R |
| Immunogen | Human CEACAM5 |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
