InVivoSIM anti-human CD40 (Sotigalimab Biosimilar)
Catalog #SIM0072
Clone:
Sotigalimab
Reactivities:
Human, Monkey
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Sotigalimab, making it ideal for research use. Sotigalimab is a humanized IgG1 monoclonal antibody that targets CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), P50, and CDW40. CD40 is a pivotal co-stimulatory receptor that is expressed by both non-immune cells (e.g., epithelial malignancies) as well as immune cells, including antigen-presenting cells such as dendritic cells, macrophages, and B lymphocytes. CD40ās interaction with its ligand CD40L (CD154) is essential to the activation of T cells (including CD8+ T cells), the maturation of B cells, and various immune system-related pathophysiological conditions. In cancers, the CD40-CD40L interaction can have different outcomes depending on the level of engagement, potentially promoting cell survival at low levels and inducing cell death at high levels. Consistent with CD40 engagement, experimental studies involving advanced solid tumors and the use of high-affinity CD40-agonistic antibody Sotigalimab have demonstrated this antibody to produce dose-dependent increases in APCs and T-cell activation. The sotigalimab biosimilar antibody is a valuable tool to explore and harness the power of CD40 signaling, immune cell activation, and anti-cancer immuno-therapeutics development.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S267E/E356D/M358L |
| Immunogen | Human CD40 |
| Reported Applications |
Functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer