InVivoSIM anti-human amyloid beta (Donanemab Biosimilar)
Catalog #SIM0048
Clone:
Donanemab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Donanemab, making it ideal for research use. Donanemab is a humanized IgG1Īŗ anti-human APP antibody, and it specifically reacts with abeta 42 (AĪ²42), a pyroglutamate form of AĪ² that is aggregated in amyloid plaques during the pathogenesis of Alzheimerās disease (AD). AĪ²42 results from an aberrant processing of amyloid-Ī² protein precursor (APP or AĪ²PP). The initial cleavage of APP is caused by either Ī±- secretase or Ī²-secretase (or BACE1). sAPPĪ± and the Ī±-C-terminal fragment (Ī±-CTF or APP-C83) are produced by Ī±-secretase cleavage of APP, whereas sAPPĪ² and the Ī²-C-terminal fragment (Ī²-CTF or APP-C99) are produced by Ī²-secretase cleavage. Ī³-secretase further cleaves C83 and C99, yielding the P3 peptide and AĪ², respectively. Depending on the location of the Ī³-secretase cleavage, AĪ² can have a length of 37-43 amino acids in its peptide sequence. The two main AĪ² species are AĪ²42 and AĪ²40. In early AD pathology, AĪ² species play a critical role by seeding toxic oligomeric species with AĪ²42 being more preferentially depositing in amyloid plaques than AĪ²40. Donanemab specifically recognizes AĪ² p3-42 (AĪ²42) and it is reported to impede the progression of AD by clearing plaques through microglial-mediated phagocytosis in mice without causing microhemorrhages. Donanemab biosimilar antibodies are commonly used for in vitro and in vivo studies involving Alzheimer's disease and Down syndrome experimental models.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human APP |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
