Catalog #SIM0048
InVivoSIM anti-human amyloid beta (Donanemab Biosimilar)
Clone
Donanemab
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Donanemab, making it ideal for research use. Donanemab is a humanized IgG1κ anti-human APP antibody, and it specifically reacts with abeta 42 (Aβ42), a pyroglutamate form of Aβ that is aggregated in amyloid plaques during the pathogenesis of Alzheimer’s disease (AD). Aβ42 results from an aberrant processing of amyloid-β protein precursor (APP or AβPP). The initial cleavage of APP is caused by either α- secretase or β-secretase (or BACE1). sAPPα and the α-C-terminal fragment (α-CTF or APP-C83) are produced by α-secretase cleavage of APP, whereas sAPPβ and the β-C-terminal fragment (β-CTF or APP-C99) are produced by β-secretase cleavage. γ-secretase further cleaves C83 and C99, yielding the P3 peptide and Aβ, respectively. Depending on the location of the γ-secretase cleavage, Aβ can have a length of 37-43 amino acids in its peptide sequence. The two main Aβ species are Aβ42 and Aβ40. In early AD pathology, Aβ species play a critical role by seeding toxic oligomeric species with Aβ42 being more preferentially depositing in amyloid plaques than Aβ40. Donanemab specifically recognizes Aβ p3-42 (Aβ42) and it is reported to impede the progression of AD by clearing plaques through microglial-mediated phagocytosis in mice without causing microhemorrhages. Donanemab biosimilar antibodies are commonly used for in vitro and in vivo studies involving Alzheimer's disease and Down syndrome experimental models.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human APP |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Western blot |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |