InVivoMAb anti-rat CD11b/c (OX42)

Catalog #BE0473
Clone:
OX-42
Reactivities:
Rat

$172.00 - $4,494.00

$172.00 - $4.00

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  • 100 mg - $4,494.00
  • 50 mg - $3,175.00
  • 25 mg - $2,109.00
  • 5 mg - $630.00
  • 1 mg - $172.00
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Product Details

The OX-42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b and CD11c are expressed on monocytes, macrophages, dendritic cells, NK cells, granulocytes, and microglial cells. The CD11b/c antigens are crucial in integrin biology, playing pivotal roles in cell migration during development, wound healing, cell differentiation, and apoptosis. Epitope mapping suggests that this antibody recognizes a common epitope shared between the integrin αM (CD11b) and integrin αx (CD11c) chains of these integrin molecules. The OX-42 antibody is extensively cited to bind the CR3 complement (C3bi) receptor, a heterodimer of CD11b and CD18 (integrin beta 2), also known as CR3 and Mac-1. Ligands of the CR3 receptor include C3bi, ICAM‐1 (an intercellular adhesion molecule), and fibrinogen (a coagulation protein). CR3 is a mediator of cell adhesion and phagocytosis involved in a range of signaling pathways, notably TLR‐4 signaling in dendritic cells and B cell receptor signaling. In functional experiments, the OX-42 antibody has been shown to inhibit the formation of complement-mediated rosettes and leukocyte migration in vitro and block iC3b binding to CR3 in vitro as well as in vivo. The CD11b/c antigens are also expressed in neural tissues, and the OX-42 antibody is well-recognized for identifying resident microglial cells in the central nervous system (CNS).

Specifications

Isotype Mouse IgG2a, Īŗ
Recommended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Peritoneal macrophages of rat
Reported Applications in vivo blocking of C3bi binding to CR3
in vitro blocking of C3bi binding to CR3
in vitro functional assay
Immunoprecipitation
Flow cytometry
Immunofluorescence
Immunohistochemistry (paraffin)
Immunohistochemistry (frozen)
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <2EU/mg (<0.002EU/μg)
Determined by LAL gel clotting assay
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from cell culture supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.