Catalog #BE0473
InVivoMAb anti-rat CD11b/c (OX42)
Clone
OX-42
Reactivities
Rat
Isotype
Mouse IgG2a, κ
Product Description
The OX-42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b and CD11c are expressed on monocytes, macrophages, dendritic cells, NK cells, granulocytes, and microglial cells. The CD11b/c antigens are crucial in integrin biology, playing pivotal roles in cell migration during development, wound healing, cell differentiation, and apoptosis. Epitope mapping suggests that this antibody recognizes a common epitope shared between the integrin αM (CD11b) and integrin αx (CD11c) chains of these integrin molecules. The OX-42 antibody is extensively cited to bind the CR3 complement (C3bi) receptor, a heterodimer of CD11b and CD18 (integrin beta 2), also known as CR3 and Mac-1. Ligands of the CR3 receptor include C3bi, ICAM‐1 (an intercellular adhesion molecule), and fibrinogen (a coagulation protein). CR3 is a mediator of cell adhesion and phagocytosis involved in a range of signaling pathways, notably TLR‐4 signaling in dendritic cells and B cell receptor signaling. In functional experiments, the OX-42 antibody has been shown to inhibit the formation of complement-mediated rosettes and leukocyte migration in vitro and block iC3b binding to CR3 in vitro as well as in vivo. The CD11b/c antigens are also expressed in neural tissues, and the OX-42 antibody is well-recognized for identifying resident microglial cells in the central nervous system (CNS).
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Peritoneal macrophages of rat |
| Reported Applications |
in vivo blocking of C3bi binding to CR3 in vitro blocking of C3bi binding to CR3 in vitro functional assay Immunoprecipitation Flow cytometry Immunofluorescence Immunohistochemistry (paraffin) Immunohistochemistry (frozen) |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |