InVivoMAb anti-mouse CXCR6 (CD186)
Catalog #BE0463
Clone:
Cx6Mab-1
Reactivities:
Mouse
Product Details
The Cx6Mab-1 monoclonal antibody reacts with the N-terminal extracellular ligand-binding domain of mouse CXC chemokine receptor 6 (mCXCR6) also known as CD186, BONZO, or STRL33. CXCR6 is expressed on naive CD8+ T cells and a subset of memory CD4+ T cells, natural killer T cells (NKTs), dendritic cells (DCs), pulmonary alveolar macrophages, and innate lymphoid cells (ILCs). The ligand for CXCR6 is C-X-C chemokine CXCL16 that is predominantly expressed in DCs, monocytes, and various other tissue cells, primarily epithelial cells. The CXCR6-CL16 interaction guides immune cell homing (T lymphocyte migration to peripheral tissues) and their activation, expansion, and cytotoxic activity. Retroviruses such as simian immunodeficiency viruses (SIVs), some strains of HIV-2, and macrophage-tropic HIV-1 use CXCR6 as a coreceptor in conjunction with CD4 to enter target cells. In solid tumors, CXCR6 mediates CD8+ T-cell homing to tumor stromal perivascular niches and promotes interactions with CXCL16+ DCs and IL-15 expression for facilitating the growth of T-cells inside the tumor microenvironment. CXCR6 expression is also linked to the activation of Akt/mTOR and ERK/MAPK pathways and other downstream targets responsible for cancer growth and metastasis. CXCR6ās prognostic significance varies among malignancies, but it is regarded as a reliable biomarker to predict the response to anti-PD-1 immunotherapy in various cancers.Specifications
| Isotype | Rat IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Synthetic peptide corresponding to the N-terminal extracellular region of mouse CXCR6 (AA 1-19) |
| Reported Applications |
Flow cytometry Western blotting ELISA For details on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer