Catalog #BE0004-1FAB
InVivoMAb anti-mouse CD8α F(ab) fragment
Clone
53-6.7 f(ab) Fragments
Reactivities
Mouse
Isotype
Rat IgG2a, κ
Product Description
This product is a F(ab) fragment derived from the anti-mouse CD8α antibody clone 53-6.7. CD8α is a co-receptor expressed on cytotoxic T lymphocytes that enhances T cell receptor (TCR) signaling through interactions with MHC class I molecules. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to CD8α while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. This format is useful in experiments where CD8 targeting is desired without triggering immune effector mechanisms, cross-linking, or depletion of CD8⁺ T cells. F(ab) fragments targeting CD8α may also be useful in emerging targeted delivery strategies. For example, F(ab) fragments can be used to coat nanoparticles or other delivery systems to selectively target CD8⁺ T cells and deliver genetic payloads such as CAR constructs directly in vivo. By avoiding Fc-mediated immune interactions, F(ab) fragments can help preserve target cells while enabling receptor-specific targeting and delivery. This anti-human CD8α F(ab) fragment therefore provides a useful reagent for studies focused on CD8⁺ T cell targeting, receptor engagement without Fc effects, and targeted delivery approaches.
Specifications
| Isotype | Rat IgG2a, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 6.5 Dilution Buffer |
| Immunogen | Mouse Spleen Cells or Thymocyte Membranes |
| Formulation |
PBS, pH 6.5 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Papain Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |