Catalog #BE0486
InVivoMAb anti-mouse CCR7 (CD197)
Clone
C7Mab-2
Reactivities
Mouse
Isotype
Rat IgG2b, κ
Product Description
The C7Mab-2 monoclonal antibody reacts with extracellular loop 3 of mouse CC chemokine receptor type-7 (CCR7). The CCR7 protein is also called the MIP-3 beta receptor, EBI1, and CD197. CCR7 is expressed as a multipass transmembrane protein on a wide range of immune cells, such as naive T and B cells, central memory T cells, regulatory T cells (Tregs), natural killer (NK) cells, mature dendritic cells (DCs), plasmacytoid dendritic cells (pDCs), and some cancer cells. The primary ligands of CCR7 are CCL19 and CCL21, which are constitutively expressed in the high endothelial venules (HEVs) and lymph node parenchyma. CCR7-CCL19/CCL21 signaling promotes the migration of CCR7+ cells to secondary lymphoid organs (e.g., lymph nodes, thymus, and spleen). Experiments involving genetic knockout of CCR7 and impaired T cell migration to lymphoid organs have demonstrated the essential role of CCR7 for T cell recruitment in vivo. CCR7 is involved in the pathophysiology of cancer metastasis to lymph nodes, immuno-allergic reactions (including asthma), autoimmune diseases (e.g., rheumatoid arthritis and systemic lupus erythematosus), and infections (e.g., pneumonia, HIV-1, and malaria). Antibody-based blockade of CCR7 signaling-mediated chemotaxis is emerging as a promising strategy for experimental immunotherapy of cancers and other diseases.
NOTE: Please see Yamamoto et. al. Microbes & Immunity (doi: 10.36922/MI025130028) for the characterization data of the C7Mab-2 antibody.
NOTE: Please see Yamamoto et. al. Microbes & Immunity (doi: 10.36922/MI025130028) for the characterization data of the C7Mab-2 antibody.
Specifications
| Isotype | Rat IgG2b, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Synthetic peptides corresponding to extracellular loops of mouse CCR7 |
| Reported Applications |
Flow cytometry ELISA Immunohistochemistry (paraffin) For details on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |