InVivoMAb anti-mouse ACKR4 (CCR11)

Name: InVivoMAb anti-mouse ACKR4 (CCR11)
Brand: BE0467
SKU: BE0467
Availability: InStock

Clone A4Mab-3

Reactivities Mouse

Isotype Rat IgG2b, κ

Certificate of Analysis Technical Data Sheet Safety Data Sheet Citations and References

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$172.00 - $4,494.00

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Product Description

The A4Mab-3 monoclonal antibody reacts with mouse atypical chemokine receptor 4 (ACKR4), also known as CCR11, CCRL1, CCX CKR, PPR1, and CCBP2. ACKR4 is expressed on T cells, stromal cells, and a subset of lymphatic endothelial cells within the skin, spleen, and gut. ACKR4 is a seven-transmembrane domain-containing protein that belongs to the atypical chemokine receptors (ACKRs) family. Due to their function, ACKRs are considered chemokine decoy receptors, internalizing receptors (interceptors), or chemokine-scavenging receptors. They mediate β-arrestin-dependent internalization of chemokines, followed by lysosomal degradation of the receptor-ligand complex. ACKR4 plays a key role in regulating cell migration by reducing the availability of inflammatory chemokines, specifically CCL19, CCL22, and CCL25, thereby limiting migratory responses mediated by CCR7, CCR6, CCR4, and CCR9. ACKR4 negatively regulates CXCR3-induced chemotaxis and facilitates the homing of CC7+ dendritic cells through scavenging and shaping CCL19 and CCL21 gradients in lymph nodes. ACKR4 is also involved in the regulation of thymic T-cell development and suppression of spontaneous autoimmunity. Loss of ACKR4 in colorectal cancer in mice is reported to impair DC migration to tumor-draining lymph nodes, which leads to a reduced number of tumor-specific T cells and resistance to immune checkpoint blockade therapy.

Specifications

Isotype	Rat IgG2b, κ
Recommended Isotype Control(s)	InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Immunogen	A synthetic peptide corresponding to the N-terminal extracellular region of mACKR4 (amino acids 1-19)
Reported Applications	Flow cytometry ELISA For details on in vivo applications, please contact technicalservice@bioxcell.com
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	≤1EU/mg (≤0.001EU/μg) Determined by LAL gel clotting assay
Purity	≥95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Need a Custom Formulation?	See All Antibody Customization Options

Application References

Flow Cytometry

ELISA

Hirose M, Suzuki H, Ubukata R, Tanaka T, Kaneko MK, Kato Y. (2024). "Development of specific anti-mouse atypical chemokine receptor 4 monoclonal antibodies" Biochem Biophys Rep .

PubMed

Leukocyte migration is an essential function of innate and adaptive immune responses. Chemokines and their receptors control the migration system. The abundance of chemokines is controlled by atypical chemokine receptors (ACKRs), chemokine receptor-like molecules that do not couple to the G protein signaling pathways. Among them, ACKR4 regulates dendritic cell migration by controlling the ligands and is involved in tumor development in mouse models. Because no anti-mouse ACKR4 (mACKR4) monoclonal antibody (mAb) for flow cytometry has been reported, this study aimed to develop a novel mAb for mACKR4. Among the established anti-mACKR4 mAbs, A₄Mab-1 (rat IgG_2b, kappa), A₄Mab-2 (rat IgG_2b, kappa), and A₄Mab-3 (rat IgG_2b, kappa) recognized mACKR4-overexpressed Chinese hamster ovary-K1 (CHO/mACKR4) by flow cytometry. The dissociation constant (K _D) values of A₄Mab-1, A₄Mab-2, and A₄Mab-3 for CHO/mACKR4 were determined as 6.0 × 10^-9 M, 1.3 × 10^-8 M, and 1.7 × 10^-9 M, respectively. Furthermore, A₄Mab-1 and A₄Mab-2 could detect mACKR4 by western blotting. These results indicated that A₄Mab-1, A₄Mab-2, and A₄Mab-3 help to detect mACKR4 by flow cytometry and western blotting and obtain the proof of concept in preclinical models.