InVivoMAb anti-mouse ACKR4 (CCR11)
Product Details
The A4Mab-3 monoclonal antibody reacts with mouse atypical chemokine receptor 4 (ACKR4), also known as CCR11, CCRL1, CCX CKR, PPR1, and CCBP2. ACKR4 is expressed on T cells, stromal cells, and a subset of lymphatic endothelial cells within the skin, spleen, and gut. ACKR4 is a seven-transmembrane domain-containing protein that belongs to the atypical chemokine receptors (ACKRs) family. Due to their function, ACKRs are considered chemokine decoy receptors, internalizing receptors (interceptors), or chemokine-scavenging receptors. They mediate Ī²-arrestin-dependent internalization of chemokines, followed by lysosomal degradation of the receptor-ligand complex. ACKR4 plays a key role in regulating cell migration by reducing the availability of inflammatory chemokines, specifically CCL19, CCL22, and CCL25, thereby limiting migratory responses mediated by CCR7, CCR6, CCR4, and CCR9. ACKR4 negatively regulates CXCR3-induced chemotaxis and facilitates the homing of CC7+ dendritic cells through scavenging and shaping CCL19 and CCL21 gradients in lymph nodes. ACKR4 is also involved in the regulation of thymic T-cell development and suppression of spontaneous autoimmunity. Loss of ACKR4 in colorectal cancer in mice is reported to impair DC migration to tumor-draining lymph nodes, which leads to a reduced number of tumor-specific T cells and resistance to immune checkpoint blockade therapy.Specifications
| Isotype | Rat IgG2b, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | A synthetic peptide corresponding to the N-terminal extracellular region of mACKR4 (amino acids 1-19) |
| Reported Applications |
Flow cytometry ELISA For details on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
Flow Cytometry, ELISA
Hirose M, Suzuki H, Ubukata R, Tanaka T, Kaneko MK, Kato Y. (2024). "Development of specific anti-mouse atypical chemokine receptor 4 monoclonal antibodies" Biochem Biophys Rep . PubMed
Leukocyte migration is an essential function of innate and adaptive immune responses. Chemokines and their receptors control the migration system. The abundance of chemokines is controlled by atypical chemokine receptors (ACKRs), chemokine receptor-like molecules that do not couple to the G protein signaling pathways. Among them, ACKR4 regulates dendritic cell migration by controlling the ligands and is involved in tumor development in mouse models. Because no anti-mouse ACKR4 (mACKR4) monoclonal antibody (mAb) for flow cytometry has been reported, this study aimed to develop a novel mAb for mACKR4. Among the established anti-mACKR4 mAbs, A4Mab-1 (rat IgG2b, kappa), A4Mab-2 (rat IgG2b, kappa), and A4Mab-3 (rat IgG2b, kappa) recognized mACKR4-overexpressed Chinese hamster ovary-K1 (CHO/mACKR4) by flow cytometry. The dissociation constant (K D) values of A4Mab-1, A4Mab-2, and A4Mab-3 for CHO/mACKR4 were determined as 6.0 Ć 10-9 M, 1.3 Ć 10-8 M, and 1.7 Ć 10-9 M, respectively. Furthermore, A4Mab-1 and A4Mab-2 could detect mACKR4 by western blotting. These results indicated that A4Mab-1, A4Mab-2, and A4Mab-3 help to detect mACKR4 by flow cytometry and western blotting and obtain the proof of concept in preclinical models.