InVivoMAb anti-human/monkey CXCR4 (CD184)
Catalog #BE0472
Clone:
12G5
Reactivities:
Human, Monkey
Product Details
The 12G5 monoclonal antibody reacts with mouse CXC chemokine receptor 4 (CXCR4), also called fusin and CD184. CXCR4 is a hepta-transmembrane domain G protein-coupled receptor (GPCR) that is expressed in hematopoietic cells, endothelial cells, neurons, and embryonic as well as adult stem cells. The primary ligand of CXCR4 is CXCL12, also known as stromal cell-derived factor-1Ī± (SDF-1Ī±). Additionally, CXCR4 is reported to act as a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2. The CXCR4-CXCL12 signaling pathway plays a critical role in activating multiple signaling pathways including ERK1/2, ras, p38 MAPK, PLC/MAPK, and SAPK/JNK. CXCR4-CXCL12 signaling also regulates several biological processes including cell survival, proliferation, migration, and stemness. Overexpression of CXCR4 is often linked to poor prognosis in various cancers, and during cancer metastasis, CXCR4 positive cancer cells are chemotactically homed to tissues such as the liver, bone marrow, lungs, and lymph nodes that express abundant levels of CXCL12. CXCR4 as well as CXCR4/CXCL12 antagonists are emerging as attractive targets for experimental cancer therapeutics. The 12G5 antibody binds a region formed by the second extracellular loop (ECL2) of CXCR4, and several CXCR4 antagonists that block the binding of CXCL12 to CXCR4 are known to compete with this antibody. In in vitro and in vivo studies, the 12G5 is demonstrated to block the cognate ligand, CXCL12/SDF-1, and gp120/160 from binding to CXCR4.Specifications
| Isotype | Mouse IgG2a, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | CP-MAC-infected Sup-T1 cells |
| Reported Applications |
in vivo blocking of CXCR4 in vitro blocking of CXCR4 in vivo imaging in vitro functional assay Immunoprecipitation flow cytometry immunofluorescence Immunohistochemistry (paraffin) Immunohistochemistry (frozen) |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb mouse IgG2a isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
