Imaging resident and recruited macrophage contribution to Wallerian degeneration
Imaging resident and recruited macrophage contribution to Wallerian degeneration
Authors: Alexandre Boissonnas, Floriane Louboutin, Marie Laviron, Pierre-Louis Loyher, Elodie Reboussin, Sandrine Barthelemy, Annabelle RĆ©aux-Le Goazigo, Christian S. Lobsiger, BĆ©hazine CombadiĆØre, StĆ©phane MĆ©lik Parsadaniantz, Christophe CombadiĆØre
Abstract
Wallerian degeneration (WD) is a process of autonomous distal degeneration of axons upon injury. Macrophages (MPs) of the peripheral nervous system (PNS) are the main cellular agent controlling this process. Some evidence suggests that resident PNS-MPs along with MPs of hematogenous origin may be involved, but whether these two subsets exert distinct functions is unknown. Combining MP-designed fluorescent reporter mice and coherent antiāStokes Raman scattering (CARS) imaging of the sciatic nerve, we deciphered the spatiotemporal choreography of resident and recently recruited MPs after injury and unveiled distinct functions of these subsets, with recruited MPs being responsible for efficient myelin stripping and clearance and resident MPs being involved in axonal regrowth. This work provides clues to tackle selectively cellular processes involved in neurodegenerative diseases.
Graphical Abstract
Reference: Alexandre Boissonnas, Floriane Louboutin, Marie Laviron, Pierre-Louis Loyher, Elodie Reboussin, Sandrine Barthelemy, Annabelle RĆ©aux-Le Goazigo, Christian S. Lobsiger, BĆ©hazine CombadiĆØre, StĆ©phane MĆ©lik Parsadaniantz, Christophe CombadiĆØre; Imaging resident and recruited macrophage contribution to Wallerian degeneration.Ā J Exp MedĀ 2 November 2020; 217 (11): e20200471. doi:Ā https://doi.org/10.1084/jem.20200471
Product Highlights:
The authors used Bio X Cell's anti-mouse CSF1R (CD115) (clone AFS98) in this research study.