Catalog #FM0021-PE
FlowMAb PE anti-mouse L-Selectin (CD62L)
Clone
Mel-14
Reactivities
Mouse
Isotype
Rat IgG2a, κ
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Product Description
The Mel-14 monoclonal antibody reacts with mouse CD62L, also known as L-selectin and MEL-14. CD62L is a 76 kDa glycoprotein and a member of the selectin family that is expressed by neutrophils, monocytes, the majority of naïve T and B cells, a subset of memory T cells, NK cells, and most thymocytes. This protein undergoes a shedding process upon cellular activation wherein CD62L is actively cleaved from the surface of a cell membrane by ADAM17. CD62L binds to many glycoprotein ligands, including CD34, GlyCAM-1, MAdCAM-1, and PSGL-1, and acts as a “homing receptor” for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. This R-phycoerythrin (R-PE or PE)-conjugated version of the Mel-14 antibody is useful for flow cytometry, and immunohistochemistry (frozen) applications.
Specifications
| Isotype | Rat IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | FlowMAb PE rat IgG2a isotype control, anti-trinitrophenol |
| Conjugation | PE |
| Excitation Source | Yellow-Green 488 nm, 532 nm, 561 nm |
| Excitation Max | 496 nm, 566 nm |
| Emission Max | 576 nm |
| Immunogen | C3H/eb mouse B lymphoma 38C-13 |
| Protocol Information | It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. |
| Concentration | 0.2 mg/ml |
| Formulation |
PBS, pH 7.0 Contains 0.09% Sodium Azide |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G. Conjugated with R-phycoerythrin under optimal conditions. |
| Storage | The antibody solution should be stored at the stock concentration at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |