FlowMAb FITC anti-mouse L-Selectin (CD62L)
Catalog #FM0021-FITC
Clone:
Mel-14
Reactivities:
Mouse
Product Details
The Mel-14 monoclonal antibody reacts with mouse CD62L, also known as L-selectin and MEL-14. CD62L is a 76 kDa glycoprotein and a member of the selectin family that is expressed by neutrophils, monocytes, the majority of naĆÆve T and B cells, a subset of memory T cells, NK cells, and most thymocytes. This protein undergoes a shedding process upon cellular activation wherein CD62L is actively cleaved from the surface of a cell membrane by ADAM17. CD62L binds to many glycoprotein ligands, including CD34, GlyCAM-1, MAdCAM-1, and PSGL-1, and acts as a āhoming receptorā for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. This fluorescein isothiocyanate (FITC)-conjugated version of the Mel-14 antibody is useful for flow cytometry, and immunohistochemistry (frozen) applications.Specifications
| Isotype | Rat IgG2a,Ā Īŗ |
|---|---|
| Recommended Isotype Control(s) | FlowMAb FITC rat IgG2a isotype control, anti-trinitrophenol |
| Conjugation | FITC |
| Excitation Source | Blue 488 nm |
| Excitation Max | 494 nm |
| Emission Max | 518 nm |
| Immunogen | C3H/eb mouse B lymphoma 38C-13 |
| Formulation |
PBS, pH 7.0 Contains 0.09% Sodium Azide |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G. Conjugated with fluorescein isothiocyanate under optimal conditions. |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C and protected from prolonged exposure to light. Do not freeze. |
Additional Formats
Recommended Products
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Recommended Isotype Control(s)
FlowMAb FITC rat IgG2a isotype control, anti-trinitrophenol
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer