Catalog #RP033
Recombinant zebrafish FGF-2 protein
Reactivities
Zebrafish, Human
Applications
Stem cell expansion and maintenance
Product Description
Recombinant zebrafish FGF-2 protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family, a group of 18–22 members grouped into 6–7 subfamilies that regulate cell proliferation, survival, migration and differentiation. FGF-2 is one of the FGFs that binds to and signals through all four tyrosine kinase FGF receptors (FGFR1–4), which recruit SH2- and PTB-domain signaling proteins to activate the RAS/MAP kinase, PI3 kinase/Akt and PLCγ pathways. FGF family members have critical roles in vertebrate and invertebrate embryonic development, and in adult cells they modulate tissue maintenance, wound healing and angiogenesis, while FGF-2 itself regulates cell growth, survival and proliferation. FGF-2 is an essential growth factor for maintaining pluripotency in embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), acting through PI3 kinase/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activation of activin A, and in feeder culture systems it stimulates production of essential growth factors and cytokines from the feeder layer, making it a key reagent for stem cell culture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: zebrafish. Cross-reactivity: human.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: zebrafish. Cross-reactivity: human.
Specifications
| Reported Applications | Stem cell expansion and maintenance |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |