Catalog #RP096
Recombinant tuna FGF-2 (154 aa) protein
Reactivities
Fish
Applications
Expansion of tuna pluripotent, embryonic and mesenchymal stem cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Product Description
Recombinant tuna FGF-2 (154 aa) protein (fibroblast growth factor 2), also known as basic fibroblast growth factor (bFGF), is a 154-amino-acid, 17 kDa member of the fibroblast growth factor family that plays pivotal roles in cell growth, differentiation and angiogenesis and is a significant growth factor in embryonic development, tissue repair and wound healing. FGF-2 comprises multiple isoforms and adopts a β-trefoil fold stabilized by disulfide bonds, a structure essential for its biological activity. As a core component of stem cell culture media, FGF-2 maintains the pluripotency and self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) by activating signaling pathways such as the mitogen-activated protein kinase (MAPK) pathway, which sustains expression of pluripotency-associated genes including Oct4, Sox2 and Nanog, prevents differentiation and allows expansion in vitro. It can also be incorporated into scaffolds or hydrogels together with stem cells to promote proliferation and differentiation within a three-dimensional environment, making tuna FGF-2 a valuable reagent for cultivated tuna protocols and serum-free media development in the cellular agriculture industry.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: tuna.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: tuna.
Specifications
| Reported Applications |
Expansion of tuna pluripotent, embryonic and mesenchymal stem cells Cellular agriculture and cultivated meat cell culture media optimization Serum-free media development Cellular proliferation, migration and survival |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |