Catalog #RP095
Recombinant tuna FGF-2 (145 aa) protein
Reactivities
Fish
Applications
Expansion of tuna pluripotent, embryonic and mesenchymal stem cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Product Description
Recombinant tuna FGF-2 (145 aa) protein, also known as basic fibroblast growth factor (bFGF), is a key member of the fibroblast growth factor family, a 145-amino-acid protein of around 16.2 kDa characterized by a disulfide-stabilized β-trefoil fold essential for its biological activity. FGF-2 plays pivotal roles in cell growth, differentiation and angiogenesis and is a significant growth factor in embryonic development, tissue repair and wound healing. In stem cell research, FGF-2 is a key component of culture media for maintaining the pluripotency and self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), activating signaling pathways including MAPK to sustain expression of pluripotency-associated genes such as Oct4, Sox2 and Nanog, preventing differentiation and enabling in vitro expansion. FGF-2 can also be incorporated into scaffolds or hydrogels with stem cells to promote their proliferation and differentiation in a three-dimensional environment, and it holds great promise for the development of cultivated tuna protocols and serum-free media, making it a key reagent for cellular agriculture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: tuna.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: tuna.
Specifications
| Reported Applications |
Expansion of tuna pluripotent, embryonic and mesenchymal stem cells Cellular agriculture and cultivated meat cell culture media optimization Serum-free media development Cellular proliferation, migration and survival |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16.2 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |