Catalog #RP093
Recombinant salmon FGF-2 (145 aa) protein
Reactivities
Fish
Applications
Expansion of salmon pluripotent, embryonic and mesenchymal stem cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Product Description
Recombinant salmon FGF-2 (145 aa) protein (fibroblast growth factor 2), also known as basic fibroblast growth factor (bFGF), is a 145-amino-acid, 16.1 kDa member of the fibroblast growth factor family, characterized by a β-trefoil fold stabilized by disulfide bonds that is essential for its biological activity, and it plays pivotal roles in cell growth, differentiation and angiogenesis. FGF-2 is a significant growth factor in embryonic development, tissue repair and wound healing, and it exists as multiple isoforms. In stem cell research, FGF-2 is a key component of culture media for maintaining the pluripotency and self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), activating signaling pathways including the MAPK pathway to sustain expression of the pluripotency-associated genes Oct4, Sox2 and Nanog, preventing differentiation and enabling expansion in vitro. FGF-2 can also be incorporated into scaffolds or hydrogels with stem cells to promote their proliferation and differentiation in a three-dimensional environment, making salmon FGF-2 a promising reagent for cultivated salmon protocols and serum-free media development in cellular agriculture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: salmon.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: salmon.
Specifications
| Reported Applications |
Expansion of salmon pluripotent, embryonic and mesenchymal stem cells Cellular agriculture and cultivated meat cell culture media optimization Serum-free media development Cellular proliferation, migration and survival |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16.1 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |