Catalog #RP042
Recombinant rat/mouse FGF-10 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Differentiation of embryonic stem cells into gut-like structures, cardiomyocytes and hepatocytes
Epithelial to mesenchymal transition
Epithelial to mesenchymal transition
Product Description
Recombinant rat/mouse FGF-10 protein (fibroblast growth factor 10) is a ~20 kDa mesenchyme-secreted growth factor, closely related to FGF-7 and bearing a serine-rich N-terminal region, that is bound and activated by extracellular FGF-BP and signals through the epithelial FGF receptor 2b (FGFR2b), with weaker binding to FGFR1b. During development, FGF-10 maintains FGF signaling with epithelial FGF-8 in the limb bud mesoderm, drives mesenchyme accumulation and induces the apical ectodermal ridge, and mediates the epithelial–mesenchymal signaling from foregut mesenchyme to epithelium that underlies lung development, and it contributes to the formation of white adipose tissue, heart, liver, brain, kidney, thymus, inner ear, tongue, trachea, eye, prostate, salivary and mammary glands. In cell culture, recombinant FGF-10 drives differentiation of embryonic stem cells into gut-like structures, cardiomyocytes and hepatocytes and is a potent organoid factor, increasing organoid size and branching compared with other FGFs. Rat FGF-10 shares 100% homology with human FGF-10, making it a key reagent for stem cell and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: rat. Species similarity: human - 100%, mouse - 94%, bovine - 99%, porcine - 99%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: rat. Species similarity: human - 100%, mouse - 94%, bovine - 99%, porcine - 99%.
Specifications
| Reported Applications |
Differentiation of embryonic stem cells into gut-like structures, cardiomyocytes and hepatocytes Epithelial to mesenchymal transition |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |