Catalog #RP048
Recombinant rat/mouse BDNF protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Differentiation of midbrain dopaminergic neurons
Differentiation of iPSC derived neural progenitors to neurons
Generation of iPSC-derived midbrain organoids
Differentiation of iPSC derived neural progenitors to neurons
Generation of iPSC-derived midbrain organoids
Product Description
Recombinant rat/mouse BDNF protein (brain-derived neurotrophic factor) is a member of the neurotrophin family, alongside NGF and NT-3/4/5, whose mature active form is a ~13 kDa, 119-amino-acid cysteine-knot-stabilized dimer that binds TrkB with high affinity to activate MAPK, PI3K and PLC-γ signaling, and p75NTR with lower affinity to activate NF-κB. Released by neurons, glia, immune cells and skeletal muscle, BDNF supports the survival, growth and differentiation of neural stem cells and neurons, stimulates neurogenesis and dendrite and axon growth, drives synaptic plasticity underlying learning and memory, and contributes to neural repair. Impaired BDNF signaling is linked to Alzheimer’s and Parkinson’s diseases, depression, anxiety and spinal cord injury. In culture, recombinant BDNF maintains neurons and differentiates iPSC-derived neural progenitors, combining with GDNF, FGF-8 and Shh for dopaminergic neurons, with GDNF, NT-3 or IGF-1 for glutamatergic and GABAergic neurons, and with IGF-1 and NGF for cholinergic neurons, and it is required with GDNF for cortical organoid maturation. Rat BDNF shares 100% homology with human BDNF, making it a key reagent for neuroscience and neural stem cell research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: rat. Species similarity: mouse - 99%, human - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: rat. Species similarity: mouse - 99%, human - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Differentiation of midbrain dopaminergic neurons Differentiation of iPSC derived neural progenitors to neurons Generation of iPSC-derived midbrain organoids |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 14 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |