Catalog #RP103
Recombinant porcine vitronectin protein
Reactivities
Porcine
Applications
Maintenance and expansion of porcine iPSC, ESC and primary cells
Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes
Promotion of cell adhesion and migration
Organoid growth and proliferation
Stimulation of angiogenesis and vascular network development
Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes
Promotion of cell adhesion and migration
Organoid growth and proliferation
Stimulation of angiogenesis and vascular network development
Product Description
Recombinant porcine vitronectin protein is a multifunctional ~75 kDa glycoprotein of the extracellular matrix (ECM) and blood plasma, comprising an N-terminal somatomedin B domain that binds the urokinase receptor and stabilizes inactive PAI-1, a heparin-binding domain and hemopexin-like repeats that mediate interactions with integrins and other ECM components. By binding integrins such as αvβ3 and αvβ5, vitronectin activates PI3K/AKT and MAPK/ERK signaling to promote cell adhesion, migration, proliferation and survival, preventing anoikis, and it contributes to wound healing, fibrinolysis, immune regulation, angiogenesis and tumor metastasis. In cell culture, vitronectin is a coating substrate for epithelial, endothelial and certain cancer cells and a key component of defined, xeno-free systems for culture and maintenance of iPSCs and ESCs, and as a matrix scaffold it supports cell adhesion, clustering and organoid formation, including liver organoids. Vitronectin also enhances cell attachment on tissue-engineering scaffolds, prepares cells for cell therapy and compensates for serum adhesion factors in serum-free media, while recombinant production ensures batch-to-batch consistency, making it a key reagent for stem cell, organoid and regenerative medicine research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
Specifications
| Reported Applications |
Maintenance and expansion of porcine iPSC, ESC and primary cells Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes Promotion of cell adhesion and migration Organoid growth and proliferation Stimulation of angiogenesis and vascular network development |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 50.4 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at 1 mg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |