Catalog #RP085
Recombinant porcine TGF-β3 protein
Reactivities
Porcine
Applications
Induced pluripotent and embryonic stem cell differentiation and maintenance
iPSC-derived mesoderm differentiation
Culture of porcine (pig) muscle stem cells
Induction of cell migration, proliferation and extracellular matrix production
Cellular agriculture and cultivated meat cell culture media optimization
Inclusion in cultivated meat media
iPSC-derived mesoderm differentiation
Culture of porcine (pig) muscle stem cells
Induction of cell migration, proliferation and extracellular matrix production
Cellular agriculture and cultivated meat cell culture media optimization
Inclusion in cultivated meat media
Product Description
Recombinant porcine TGF-β3 protein (transforming growth factor beta 3) is a member of the TGF-β family and wider TGF-β superfamily that, like TGF-β1 and TGF-β2, signals through receptor complexes of type I and type II serine/threonine kinase receptors to activate intracellular SMAD transcription factors. The TGF-β family shows a high degree of overlap and redundancy in regulating cell survival, proliferation and differentiation, while TGF-β3 has isoform-specific roles in embryonic palate fusion and wound healing. In culture, TGF-β3 is often used for porcine pluripotent cell maintenance and is a component of B8 medium, the low-cost, weekend-free iPSC medium developed in the lab of Paul Burridge at Northwestern University, where TGF-β3 was shown to maintain iPSCs at as little as 0.1 ng/ml compared with 2 ng/ml for TGF-β1, making it a cost-effective alternative to TGF-β1 and a promising reagent for cultivated porcine protocols and serum-free media development in cellular agriculture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
Specifications
| Reported Applications |
Induced pluripotent and embryonic stem cell differentiation and maintenance iPSC-derived mesoderm differentiation Culture of porcine (pig) muscle stem cells Induction of cell migration, proliferation and extracellular matrix production Cellular agriculture and cultivated meat cell culture media optimization Inclusion in cultivated meat media |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |