Catalog #RP071
Recombinant porcine HGF NK1 protein
Reactivities
Porcine
Applications
Differentiation of iPSCs to hepatocyte-like cells
iPSC and primary cell differentiation to muscle
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture process development
iPSC and primary cell differentiation to muscle
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture process development
Product Description
Recombinant porcine HGF NK1 protein (hepatocyte growth factor NK1) is the smallest naturally occurring splice variant of HGF, comprising the N-terminus and first kringle domain, from a growth factor that regulates cell growth, motility and morphogenesis and plays a central role in angiogenesis, tumorigenesis and tissue regeneration. Full-length HGF is secreted as a single inactive polypeptide that is cleaved by serine proteases into a 62 kDa heavy chain and a 32–36 kDa light chain, linked by a disulfide bond to form the active heterodimer, and it binds and activates the receptor tyrosine kinase c-Met to trigger PI3K/AKT, FAK, JNK and ERK1/2 signaling. Although porcine HGF is similar in sequence to human and bovine HGF, it differs in structure and bioactivity, showing greater resistance to protease digestion and higher potency in inducing muscle cell proliferation and differentiation. These properties give porcine HGF important implications for cellular agriculture, offering a faster route toward tissues with improved regenerative qualities, and this protein is produced in an animal-free microbial system compatible with production at scale, making it a valuable growth factor for cultivated meat media development.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
Specifications
| Reported Applications |
Differentiation of iPSCs to hepatocyte-like cells iPSC and primary cell differentiation to muscle Cellular agriculture and cultivated meat cell culture media optimization Cellular agriculture process development |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 20 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |