Catalog #RP073
Recombinant porcine EGF protein
Reactivities
Porcine
Applications
Cellular agriculture process development
Inclusion in cultivated meat media
Culture of porcine (pig) muscle stem cells
Culture of porcine (pig) organoids
Differentiation of iPSCs into epithelial cells
Inclusion in cultivated meat media
Culture of porcine (pig) muscle stem cells
Culture of porcine (pig) organoids
Differentiation of iPSCs into epithelial cells
Product Description
Recombinant porcine EGF protein (epidermal growth factor) is a 6.3 kDa, 53-amino-acid globular growth factor of the EGF family, which includes TGF-alpha, amphiregulin, betacellulin, epiregulin, HB-EGF, epigen and the neuregulins, stabilized by three intramolecular disulfide bonds and released from type I transmembrane precursors by proteolytic cleavage. EGF binds high-affinity receptor tyrosine kinases of the EGFR/ErbB family (EGFR, ErbB2, ErbB3, ErbB4), triggering receptor dimerization and PI3K, ERK1/2, JAK/STAT, β-catenin and calcium signaling to stimulate proliferation, differentiation and survival of epidermal and epithelial cells, and it contributes to angiogenesis, epithelial development, wound healing and cancer progression. In culture, recombinant EGF supports expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages from iPSCs and ESCs and is a key organoid media component with R-spondin 1, Noggin or Gremlin, Wnt3a and FGF10 for intestine, stomach, liver, pancreas, brain and cancer organoids. Porcine EGF is also crucial for cultivated meat, maintaining and stabilizing pig muscle stem cells long term with preserved myogenic marker expression, making it a key reagent for organoid, stem cell and cellular agriculture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: porcine.
Specifications
| Reported Applications |
Cellular agriculture process development Inclusion in cultivated meat media Culture of porcine (pig) muscle stem cells Culture of porcine (pig) organoids Differentiation of iPSCs into epithelial cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 6.3 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |