Catalog #RP044
Recombinant mouse TGF-β3 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Expansion and differentiation of mouse pluripotent stem cells
Propagation of mouse embryonic stem cells
iPSC-derived mesoderm differentiation
Propagation of mouse embryonic stem cells
iPSC-derived mesoderm differentiation
Product Description
Recombinant mouse TGF-β3 protein (transforming growth factor beta 3) is a member of the TGF-β family and wider TGF-β superfamily that, like TGF-β1 and TGF-β2, signals through receptor complexes of type I and type II serine/threonine kinase receptors to activate intracellular SMAD transcription factors. The TGF-β isoforms show a high degree of overlap and redundancy in regulating cell survival, proliferation and differentiation, while TGF-β3 has isoform-specific roles in embryonic palate fusion and wound healing. In cell culture, TGF-β3 is widely used for pluripotent stem cell maintenance and is a component of B8 medium, the low-cost, weekend-free iPSC medium developed in the lab of Paul Burridge at Northwestern University, where Kuo et al. showed that TGF-β3 maintains iPSCs at concentrations as low as 0.1 ng/ml compared with 2 ng/ml for TGF-β1. With 100% homology to human TGF-β3, recombinant mouse TGF-β3 offers a potent, cost-effective alternative to TGF-β1, making it a valuable reagent for pluripotent stem cell culture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 100%, rat - 99%, bovine - 99%, porcine - 98%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 100%, rat - 99%, bovine - 99%, porcine - 98%.
Specifications
| Reported Applications |
Expansion and differentiation of mouse pluripotent stem cells Propagation of mouse embryonic stem cells iPSC-derived mesoderm differentiation |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 12.7 kDa (monomer) 25.4 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |