Catalog #RP080
Recombinant mouse SCF protein
Reactivities
Mouse, Human, Rat
Applications
Maintenance and differentiation of hematopoietic stem cells
Differentiation of induced pluripotent stem cells to microglia
Differentiation of induced pluripotent stem cells to microglia
Product Description
Recombinant mouse SCF protein (stem cell factor), also known as kit ligand or mast cell growth factor, is a pivotal regulator of stem cell function that signals through c-kit, a transmembrane tyrosine kinase receptor expressed on hematopoietic stem cells, neural crest-derived cells, melanocytes and germ cells, activating the Ras/MAPK, PI3K/Akt and JAK/STAT pathways that control proliferation, survival, migration and differentiation. SCF can be proteolytically cleaved near the membrane to release a soluble form that retains c-kit binding and acts as a paracrine or autocrine factor. In hematopoiesis, SCF is critical for the maintenance, expansion and self-renewal of hematopoietic stem cells (HSCs) in the bone marrow and for their differentiation into red blood cells, mast cells and platelets, and during embryogenesis it supports the proliferation, migration and survival of neural crest cells that give rise to neurons, glia, melanocytes and smooth muscle cells. Aberrant SCF/c-kit signaling contributes to gastrointestinal stromal tumors (GISTs), acute myeloid leukemia (AML) and melanoma, making recombinant SCF a key reagent for hematopoietic stem cell, developmental biology and cancer research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 95%, rat - 97%, bovine - 87%, porcine - 88%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 95%, rat - 97%, bovine - 87%, porcine - 88%.
Specifications
| Reported Applications |
Maintenance and differentiation of hematopoietic stem cells Differentiation of induced pluripotent stem cells to microglia |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 18.4 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |