Catalog #RP047
Recombinant mouse/rat NT-3 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Neural organoid growth and differentiation
Neural stem cell proliferation and neuronal differentiation
Neural stem cell research
Neural stem cell proliferation and neuronal differentiation
Neural stem cell research
Product Description
Recombinant mouse/rat NT-3 protein (neurotrophin 3) is a member of the neurotrophin family, alongside NGF, BDNF and NT-4/5, and of the cysteine knot growth factor family, in which six conserved cysteines form three intertwined disulfide bonds; bioactive NT-3 is a non-covalently linked 27.3 kDa homodimer of 13.6 kDa monomers, processed from a 257-residue precursor to a 119-residue mature protein that is identical in mice, rats, humans and pigs. NT-3 signals primarily through TrkC, with the low-affinity p75 neurotrophin receptor and, in certain cells, TrkA and TrkB. It supports sensory neuron growth, survival and connectivity during development, maintains and repairs sensory neurons in the adult, promotes survival and differentiation of peripheral, sensory and spinal motor neurons and oligodendrocytes, and contributes to heart development, and it is studied in neurodegeneration, spinal cord and nerve injury, depression and anxiety. In culture, NT-3 is used with BDNF, GDNF and IGF-1 to differentiate iPSCs into GABAergic and glutamatergic cortical neurons, is required for cortical organoid maturation, and drives oligodendrocyte differentiation with PDGF-AA and IGF-1, making it a key reagent for neuroscience research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 98%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 98%, porcine - 100%.
Specifications
| Reported Applications |
Neural organoid growth and differentiation Neural stem cell proliferation and neuronal differentiation Neural stem cell research |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 27.3 kDa (dimer) 13.7 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |