Catalog #RP045
Recombinant mouse/rat FGF-8a protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Differentiation of midbrain dopaminergic neurons
Differentiation of iPSC derived neural progenitors to neurons
Differentiation of iPSC derived neural progenitors to neurons
Product Description
Recombinant mouse/rat FGF-8a protein (fibroblast growth factor 8a) is a heparin-binding, monomeric isoform of FGF-8 with a molecular weight of 21.3 kDa and 182 residues spanning the signal sequence, N-terminal domain, FGF domain and proline-rich C-terminus, folded into two antiparallel β-sheets and several α-helices. Its conserved heparin-binding domain binds cell-surface heparan sulfate proteoglycans to localize FGF-8a to target cells, and it signals through FGF receptors to activate the Ras/MAPK pathway, with human and mouse FGF-8a sharing 100% homology. Originally discovered as a growth factor for androgen-dependent mouse mammary carcinoma cells, FGF-8a is widely expressed during embryogenesis, drives epithelial-mesenchymal transitions, organizes gastrulation and patterns the brain, eye, ear, limb and heart, and is required for morphogenesis and neurogenesis, expanding the midbrain in transgenic mice. In cell culture, FGF-8a is used to generate ESC- and iPSC-derived ventral midbrain dopaminergic neurons and supports neuronal induction and neurite outgrowth from spiral ganglion neurons, making it a key reagent for neural development and stem cell differentiation research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Differentiation of midbrain dopaminergic neurons Differentiation of iPSC derived neural progenitors to neurons |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 21.3 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |