Catalog #RP037
Recombinant mouse/rat BMP-2 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Generation of iPSC-derived osteoblasts and chondrocytes
Growth and proliferation of chondrocytes, fibroblasts and primary cells
Neural stem cell proliferation and neuronal differentiation
Stem cell differentiation into neural and microglial lineages
Growth and proliferation of chondrocytes, fibroblasts and primary cells
Neural stem cell proliferation and neuronal differentiation
Stem cell differentiation into neural and microglial lineages
Product Description
Recombinant mouse/rat BMP-2 protein (bone morphogenetic protein 2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily and a potent regulator of embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. Synthesized as a preproprotein comprising an N-terminal signal peptide, a 259-residue pro-domain and a 114-residue mature domain, BMP-2 is proteolytically processed to form active disulfide-linked homodimers as well as heterodimers with BMP-7. Mature BMP-2 shares 100% amino acid sequence identity between human, mouse and rat, and 85% identity with the related BMP-4, but less than 51% with other BMPs. As a strong differentiation factor, recombinant BMP-2 directs pluripotent stem cells toward extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes, and induces bone and cartilage formation in vitro and chondrogenesis in adult mesenchymal stem cells, making it a key growth factor for stem cell differentiation and bone and cartilage research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Generation of iPSC-derived osteoblasts and chondrocytes Growth and proliferation of chondrocytes, fibroblasts and primary cells Neural stem cell proliferation and neuronal differentiation Stem cell differentiation into neural and microglial lineages |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 26 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |