Catalog #RP040
Recombinant mouse/rat activin A protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Large scale stem cell expansion
Expansion and differentiation of mouse pluripotent stem cells
Expansion and differentiation of mouse pluripotent stem cells
Product Description
Recombinant mouse/rat activin A protein is an engineered, optimized form of activin A, a member of the TGF-β superfamily, that incorporates an N-terminal truncation removing a disulfide-linked extension, enabling extremely consistent high-yield manufacture with no observed change in bioactivity and making it ideal for large-scale stem cell culture processes. Activins are disulfide-linked dimers of inhibin β chains, with activin A a homodimer of inhibin βA synthesized as a larger precursor with a cleaved pro-domain, and its activity is regulated by the high-affinity inhibitor follistatin and by inhibins. In vivo, activin A regulates embryogenesis, reproductive system development, wound healing and immune responses. In culture, activin A is used with FGF-2 to maintain pluripotency of induced pluripotent and embryonic stem cells, directs differentiation into definitive endoderm as a precursor to pancreatic and liver cells, promotes neural precursors and astrocytic differentiation with CNTF and GDNF, supports mesodermal differentiation toward muscle, bone and blood cells, and is often used for organoid development and maintenance. Mouse activin A shares 100% homology with rat, human, porcine and bovine proteins, making it a versatile reagent for stem cell and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Large scale stem cell expansion Expansion and differentiation of mouse pluripotent stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 24 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |