Catalog #RP038
Recombinant mouse/rat activin A protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Expansion of induced pluripotent, embryonic and mesenchymal stem cells
Induced pluripotent stem cell culture and maintenance
iPSC-derived mesoderm differentiation
Differentiation of iPSC into endoderm
Expansion and differentiation of mouse pluripotent stem cells
Induced pluripotent stem cell culture and maintenance
iPSC-derived mesoderm differentiation
Differentiation of iPSC into endoderm
Expansion and differentiation of mouse pluripotent stem cells
Product Description
Recombinant mouse/rat activin A protein is a disulfide-linked homodimer of inhibin βA chains and a member of the transforming growth factor beta (TGF-β) superfamily, first identified for its ability to stimulate follicle-stimulating hormone (FSH) release from the pituitary and synthesized as a precursor with a signal peptide, a 250–350-residue pro-domain and a highly conserved mature domain. Activin A binds type I (ALK4 or ALK7) and type II (ActRIIA or ActRIIB) receptors to drive SMAD-dependent transcription, and its activity is tightly regulated by follistatin and inhibins in a feedback loop. It regulates embryonic development, organ patterning, immune responses and wound healing, and impaired signaling is associated with cancer, inflammation and fibrosis. In culture, activin A is used with FGF-2 to maintain pluripotency of iPSCs and ESCs, directs differentiation into definitive endoderm for pancreatic and liver lineages, promotes neural precursors and astrocytic differentiation with CNTF and GDNF, supports mesodermal differentiation into muscle, bone and blood cells, and is widely used in organoid culture, and because mouse activin A shares 100% homology with rat, human, porcine and bovine proteins, it is a versatile reagent for stem cell and organoid research across species.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat. Species similarity: human - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Expansion of induced pluripotent, embryonic and mesenchymal stem cells Induced pluripotent stem cell culture and maintenance iPSC-derived mesoderm differentiation Differentiation of iPSC into endoderm Expansion and differentiation of mouse pluripotent stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 26 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |