Catalog #RP054
Recombinant mouse noggin protein
Reactivities
Mouse, Rat
Applications
Tumor organoid culture
Maintenance of undifferentiated embryonic stem cells
Epithelial organoid culture
Stem cell differentiation into neural and microglial lineages
Maintenance of undifferentiated embryonic stem cells
Epithelial organoid culture
Stem cell differentiation into neural and microglial lineages
Product Description
Recombinant mouse noggin protein is an extracellular antagonist of the bone morphogenetic proteins (BMPs) of the TGF-β family, binding and inhibiting BMP-2, -4, -5, -7, -13 and -14 with varying affinities and thereby preventing their interaction with receptors and blocking activation of downstream signaling cascades. Through its control of BMP signaling, noggin plays a crucial role in embryonic patterning as well as in the development of the neural tube, teeth, hair follicles and the eye. In cell culture, recombinant noggin is used for the maintenance of pluripotent stem cells and the differentiation of induced pluripotent stem cells (iPSCs) toward neural lineages, and it is an essential BMP inhibitor in a large variety of organoid culture systems, including intestinal, liver and lung organoids, making it a key reagent for stem cell, neural differentiation and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse, rat.
Specifications
| Reported Applications |
Tumor organoid culture Maintenance of undifferentiated embryonic stem cells Epithelial organoid culture Stem cell differentiation into neural and microglial lineages |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 46 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |