Catalog #RP043
Recombinant mouse LIF protein
Reactivities
Mouse, Human
Applications
Propagation of mouse embryonic stem cells
Product Description
Recombinant mouse LIF protein (leukemia inhibitory factor) is a pleiotropic cytokine of the IL-6 superfamily that signals through a heterodimeric receptor composed of LIFR (gp190) and gp130, activating a number of intracellular pathways including the JAK/STAT, PI3K and MAPK signaling cascades. LIF signaling is essential for the maintenance of murine pluripotency, and recombinant LIF is therefore a core supplement for the culture and self-renewal of mouse embryonic stem cells. Although human LIF can also be used to maintain mouse embryonic stem cells, mouse LIF cannot bind the human LIF receptor and is consequently inactive on human cells, so mouse LIF offers a species-matched choice for murine systems, making recombinant mouse LIF a key reagent for mouse pluripotent stem cell culture and research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 97%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse. Species similarity: human - 97%.
Specifications
| Reported Applications | Propagation of mouse embryonic stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 20 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |