Catalog #RP059
Recombinant mouse FGF-2 protein
Reactivities
Mouse
Applications
Stem cell expansion and maintenance
Primary cell culture
Primary cell culture
Product Description
Recombinant mouse FGF-2 protein (fibroblast growth factor 2), also known as basic fibroblast growth factor (bFGF), is a member of the FGF family, which regulates cell proliferation, survival, migration and differentiation and has critical roles in embryonic development, tissue maintenance, wound healing and angiogenesis. FGF-2 is one of the FGFs that binds and signals through all four tyrosine kinase FGF receptors (FGFR1–4), which recruit SH2- or PTB-domain signaling proteins to activate the RAS/MAP kinase, PI3K/Akt and PLCγ pathways. It is an essential growth factor for maintaining pluripotency in embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), preserving stemness via PI3K/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activin A activation, and in feeder culture systems it stimulates production of essential growth factors and cytokines from the feeder layer. Together with TGF-β1, FGF-2 is required for feeder-free iPSC maintenance, making it a key reagent for stem cell culture and research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: mouse.
Specifications
| Reported Applications |
Stem cell expansion and maintenance Primary cell culture |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |