Catalog #RP024
Recombinant human TNF-alpha protein
Product Description
Recombinant human TNF-alpha protein (tumor necrosis factor-alpha) is a multifunctional cytokine central to inflammation, immunity and diverse physiological processes, produced mainly by activated macrophages and other immune cells as well as endothelial cells, fibroblasts and adipocytes. TNF-alpha signals through TNF receptor 1 (TNFR1), which drives pro-inflammatory responses, apoptosis and immune cell recruitment, and TNF receptor 2 (TNFR2), which supports tissue regeneration, immune regulation and cell survival. It initiates and amplifies inflammation by inducing cytokines such as IL-1 and IL-6 and chemokines that recruit immune cells, and it modulates the activation, proliferation and differentiation of T cells, B cells and NK cells, antigen presentation and adaptive immunity, while also influencing metabolism, angiogenesis, tissue remodeling and anti-tumor responses. Because dysregulated TNF-alpha drives rheumatoid arthritis, inflammatory bowel disease and psoriasis, it is a major therapeutic target, making recombinant TNF-alpha a key reagent for inflammation and immunology research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 78%, rat - 78%, bovine - 79%, porcine - 86%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 78%, rat - 78%, bovine - 79%, porcine - 86%.
Specifications
| Formulation | Lyophilized, white powder |
|---|---|
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17.3 kDa (non-covalent trimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |