Catalog #RP015
Recombinant human TGF-β3 protein
Product Description
Recombinant human TGF-β3 protein (transforming growth factor beta 3) is a member of the TGF-β family and superfamily that, like TGF-β1 and TGF-β2, signals through Type I and Type II serine/threonine kinase receptor complexes to activate intracellular SMAD transcription factors and regulate cell survival, proliferation and differentiation. TGF-β3 has isoform-specific roles in embryonic palate fusion and wound healing and is widely used in human pluripotent stem cell maintenance. It is a component of the low-cost, weekend-free B8 iPSC medium developed by the Burridge lab, where TGF-β3 maintains iPSCs at concentrations as low as 0.1 ng/ml, compared with 2 ng/ml for TGF-β1, making recombinant TGF-β3 a cost-effective alternative for stem cell culture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 100%, rat - 99%, bovine - 99%, porcine - 98%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 100%, rat - 99%, bovine - 99%, porcine - 98%.
Specifications
| Recommended Dilution Buffer | InVivoPure Reconstitution Buffer A |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 12.7 kDa (monomer) 25.4 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |