Catalog #RP053
Recombinant human R-spondin 1 LR5 protein
Reactivities
Human
Applications
Intestinal organoid culture
Tumor organoid culture
Tumor organoid culture
Product Description
Recombinant human R-spondin 1 LR5 protein (RSPO-1) is an engineered form of R-spondin 1, a secreted activator of the canonical Wnt signaling pathway and ligand for the leucine-rich repeat-containing G-protein coupled receptors LGR4–6, designed with higher affinity for LGR5 and lower affinity for LGR4 to specifically target Wnt-3a signaling in the LGR5+ stem cell population of intestinal organoids. The protein comprises the two cysteine-rich, furin-like domains that are essential for activity in stem cell culture, and because it is produced in E. coli it is not glycosylated, ensuring reliable production of homogeneous protein and enhanced comparability between batches, since glycosylation is not required for R-spondin 1 extracellular signaling or stability. Recombinant R-spondin 1 is used extensively in adult stem cell-derived and intestinal organoid culture, hematopoietic stem cell specification and other iPSC culture systems, and this highly pure, biologically active LGR5-selective variant offers a refined tool for dissecting Wnt signaling in stem cell niches, making it a key reagent for organoid and stem cell research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human.
Specifications
| Reported Applications |
Intestinal organoid culture Tumor organoid culture |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 13 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |