Catalog #RP064
Recombinant human OSM protein
Reactivities
Human
Applications
Maturation of hepatoblasts into hepatocyte like cells
Product Description
Recombinant human OSM protein (oncostatin M) is a 30 kDa secreted cytokine of the interleukin-6 family, which includes IL-11, CNTF and cardiotrophin-1, produced as a 252-amino-acid pre-pro-protein whose signal peptide and C-terminal domain are cleaved to yield the mature 195-amino-acid form. Unlike other family members, OSM binds the common gp130 subunit with low affinity and recruits either LIFRα or OSMRβ to form the type I or type II OSM receptor. OSM has essential functions in inflammation, neuroprotection, hematopoiesis, metabolism, early development, tumorigenesis and epithelial cell growth, and human OSM, which shares 45% sequence homology with mouse and rat OSM, 54% with bovine and 62% with porcine, has shown activity on mouse cells. In stem cell applications, animal-free OSM is used in iPSC and ESC protocols to promote maturation of hepatoblasts into hepatocyte-like cells for liver regenerative medicine research and drug and toxicology assay development, and it may maintain naïve mouse embryonic stem cells in the absence of LIF, while further uses include hematopoietic stem cell differentiation and cultured meat, making it a versatile reagent for stem cell and regenerative medicine research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 50%, rat - 50%, bovine - 60%, porcine - 68%. NB: although the sequence identity is low, human OSM has been shown to be active on mouse ES cells.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 50%, rat - 50%, bovine - 60%, porcine - 68%. NB: although the sequence identity is low, human OSM has been shown to be active on mouse ES cells.
Specifications
| Reported Applications | Maturation of hepatoblasts into hepatocyte like cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 22 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |