Catalog #RP090
Recombinant human/mouse TPO protein
Reactivities
Porcine, Mouse, Human, Rat
Applications
Differentiation of myeloid progenitors into erythrocytes and megakaryocytes
Differentiation of stem cells into erythrocytes and megakaryocytes
Maintenance and differentiation of hematopoietic stem cells
Differentiation of stem cells into erythrocytes and megakaryocytes
Maintenance and differentiation of hematopoietic stem cells
Product Description
Recombinant human/mouse TPO protein (thrombopoietin) is a class I hematopoietic cytokine of 355 amino acids, the first 155 of which are homologous to erythropoietin (EPO), that binds its receptor Mpl, expressed primarily on megakaryocytes and platelets, to regulate megakaryocyte proliferation and maturation and platelet production. TPO binding induces Mpl dimerization and JAK2 phosphorylation, activating STAT, MAPK and PI3K signaling. TPO is synthesized primarily and constitutively in the liver, and also in the kidney, spleen and bone marrow, and unlike EPO its production is not linked to anemia. Reduced platelet production causes thrombocytopenia, a common hematological disorder and frequent side effect of cancer treatment, and recombinant TPO has been investigated as a treatment shown to increase platelet counts. In culture, TPO supports ex vivo expansion of hematopoietic stem cells (HSCs) in combination with Flt3L and SCF and, in synergy with SCF and IL-3, increases the number of erythroid and myeloid progenitors, making it a key reagent for hematopoietic stem cell research and therapeutic development.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 92%, rat - 91%, bovine - 85%, porcine - 91%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 92%, rat - 91%, bovine - 85%, porcine - 91%.
Specifications
| Reported Applications |
Differentiation of myeloid progenitors into erythrocytes and megakaryocytes Differentiation of stem cells into erythrocytes and megakaryocytes Maintenance and differentiation of hematopoietic stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |