Catalog #RP020
Recombinant human/mouse TGF-β2 protein
Reactivities
Mouse, Human, Rat, Bovine
Applications
Stem cell expansion and maintenance
Epithelial to mesenchymal transition
Stimulation of protein expression and secretion
Epithelial to mesenchymal transition
Stimulation of protein expression and secretion
Product Description
Recombinant human/mouse TGF-β2 protein (transforming growth factor beta 2) is a 25.4 kDa disulfide-linked homodimer of two identical 112-amino-acid chains and a member of the TGF-β superfamily alongside TGF-β1 and TGF-β3. Released from a latent complex by thrombospondin-1, plasmin, matrix metalloproteases or integrins, TGF-β2 signals through the accessory receptor betaglycan (TGF-β RIII), the type II receptor TGF-β RII and either ALK-5 or ALK-1 to activate Smad and Smad-independent pathways. TGF-β2 regulates cell proliferation, differentiation, wound healing, apoptosis and metabolism, is essential to cardiac, lung, craniofacial, limb, eye, ear and urogenital development, and suppresses IL-2-dependent T-cell growth with roles in immunomodulation and the tumor microenvironment. In cell culture it guides stem cell fate toward chondrocytes and epithelial-like cells, drives cell migration and extracellular matrix production for wound-healing models, and is a key inducer of epithelial-mesenchymal transition (EMT), making recombinant TGF-β2 a versatile reagent for developmental, immunology and tissue-repair research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 97%, rat - 97%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 97%, rat - 97%, bovine - 100%, porcine - 100%.
Specifications
| Recommended Dilution Buffer | InVivoPure Reconstitution Buffer A |
|---|---|
| Reported Applications |
Stem cell expansion and maintenance Epithelial to mesenchymal transition Stimulation of protein expression and secretion |
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25.4 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |