Catalog #RP068
Recombinant human/mouse Shh protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Neural stem cell proliferation and neuronal differentiation
Differentiation of midbrain dopaminergic neurons
Midbrain differentiation
Corpus organoid generation
Cholinergic-like neurons (ChLNs) differentiation
Differentiation of midbrain dopaminergic neurons
Midbrain differentiation
Corpus organoid generation
Cholinergic-like neurons (ChLNs) differentiation
Product Description
Recombinant human/mouse Shh protein (Sonic Hedgehog) is a member of the Hedgehog family, alongside Indian Hedgehog (Ihh) and Desert Hedgehog (Dhh), and corresponds to SHH C24II, the bioactive N-terminal region spanning Cys24–Gly197 (Cys24Ile-Ile) that signals via canonical and non-canonical pathways through the transmembrane receptor Patched (Ptch). Shh is essential in embryonic development, tissue regeneration and tumorigenesis, regulating cell fate and patterning of the neural tube and central nervous system, including ventral forebrain, midbrain dopaminergic and hindbrain serotonergic fates, and inducing motor neurons and interneurons. Abnormal Shh signaling is associated with basal cell carcinoma, medulloblastoma and Gorlin syndrome, and its role in tissue repair makes it of interest in regenerative medicine. In cell culture, Shh is used to pattern embryos and organoids along the neural axis, to derive neural progenitors from induced pluripotent stem cells, and together with BDNF, GDNF, FGF-8, TGF-β3 or IGF-1 to generate dopaminergic and GABAergic neurons, making it a key reagent for neural stem cell and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 99%, rat - 97%, bovine - 99%, porcine - 98%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 99%, rat - 97%, bovine - 99%, porcine - 98%.
Specifications
| Reported Applications |
Neural stem cell proliferation and neuronal differentiation Differentiation of midbrain dopaminergic neurons Midbrain differentiation Corpus organoid generation Cholinergic-like neurons (ChLNs) differentiation |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 19.8 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |