Catalog #RP060
Recombinant human/mouse PDGF-AA protein
Reactivities
Mouse, Human, Rat, Bovine
Applications
Differentiation of neural progenitor cells into oligodendrocyte precursor cells
Product Description
Recombinant human/mouse PDGF-AA protein (platelet-derived growth factor AA) is a homodimeric member of the PDGF/VEGF family, produced by platelets, where it is stored in α-granules and released on activation, and by endothelial cells, fibroblasts, vascular smooth muscle cells, osteoblasts, glia and neurons. PDGFs are homo- or heterodimers of PDGF-A, -B, -C and -D chains that act through two receptors, PDGFR-α, which binds all PDGF dimers except PDGF-DD, and PDGFR-β, which binds PDGF-BB and PDGF-DD, activating downstream PI3K, MAPK and STAT3 signaling. PDGF plays important roles in embryonic development, cell proliferation, cell migration and angiogenesis, and is a potent mitogen for cells of mesenchymal origin such as fibroblasts, glial cells and vascular smooth muscle cells. In stem cell research, PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells, making it a key reagent for neural differentiation and glial biology research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 95%, rat - 95%, bovine - 91%, porcine - 89%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 95%, rat - 95%, bovine - 91%, porcine - 89%.
Specifications
| Reported Applications | Differentiation of neural progenitor cells into oligodendrocyte precursor cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 29 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |