Catalog #RP062
Recombinant human/mouse NRG-1 protein
Reactivities
Porcine, Mouse, Human, Bovine
Applications
Maintenance of pluripotency in iPSC
Product Description
Recombinant human/mouse NRG-1 protein (neuregulin 1) is a member of the neuregulin family (NRG 1–4) and consists of the β isoform of the bioactive EGF-like domain of NRG-1, the best-studied family member, whose alternative splicing and promoter usage generate six protein types (I–VI) and at least 31 isoforms with distinct tissue distribution, potency, receptor specificity and biological function. Both α and β EGF-like domain isoforms are ligands for the receptors ErbB3 (HER3) and ErbB4 (HER4), but the β isoform binds ErbB4 with higher affinity than the α isoform. NRG-1 plays an essential role in cardiac and neuronal development and is implicated in disorders such as schizophrenia and in numerous cancers, including breast cancer, where it promotes the proliferation and migration of cancer cells. In cell culture, NRG-1 is frequently used in maintenance media for human pluripotent stem cells and other cell types, making recombinant NRG-1 a valuable reagent for stem cell, cardiac and neuroscience research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 83%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 83%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications | Maintenance of pluripotency in iPSC |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 7.5 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |