Catalog #RP051
Recombinant human/mouse nodal protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
iPSC-derived mesoderm differentiation
Differentiation of iPSC into endoderm
Cancer research
Maintenance of pluripotency in iPSC
Maintenance of undifferentiated embryonic stem cells
Differentiation of iPSC into endoderm
Cancer research
Maintenance of pluripotency in iPSC
Maintenance of undifferentiated embryonic stem cells
Product Description
Recombinant human/mouse nodal protein is a member of the transforming growth factor β (TGF-β) family, which comprises the activin/inhibin, BMP and nodal subgroups, and is synthesized as a large precursor that is cleaved to a mature disulfide-linked homodimer. Nodal signals through type I and type II transmembrane serine-threonine kinase receptors to activate Smad2 and Smad3, in contrast to BMP signaling through Smad1, Smad5 and Smad8. Nodal is essential at the early epiblast stage, where it maintains expression of key pluripotency factors and, together with BMPs, controls endoderm and mesoderm specification while blocking neuroectoderm formation, and studies of gastruloids (embryonic organoids) show a role for nodal in axial patterning alongside FGF-2, DKK-1, BMP-4 and Wnt signaling. In cancer models such as glioma, recombinant nodal increases MMP-2 secretion and cell invasion. In human embryonic stem cell (hESC) culture, recombinant nodal maintains pluripotency and prevents default neuroectodermal differentiation, and together with TGF-β1 and activin A supports pluripotency under mechanical strain, making it a key reagent for stem cell, gastruloid and developmental biology research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 96%*, porcine - 97%*. *Sequences unconfirmed.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 96%*, porcine - 97%*. *Sequences unconfirmed.
Specifications
| Reported Applications |
iPSC-derived mesoderm differentiation Differentiation of iPSC into endoderm Cancer research Maintenance of pluripotency in iPSC Maintenance of undifferentiated embryonic stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25.9 kDa (homodimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |